Hotspot

trnS-GCU-trnG-UCC

LSC · 8261-8960 · 700 bp

Back to Lysimachia

Pi 0.0344

MarkerSeek score 78.70

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 700
variable_sites: 180
pi: 0.0344
unique_haplotype_count: 60
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CTTTCGTCCACTCAGCCATC	ACGAATCACACTTTTACCACT	55.0	38.1	58.6	55.5	632-801 bp	1.0	58.6
trnS-GCU-trnG-UCC_p2	CTTTCGTCCACTCAGCCATC	AGAACGAATCACACTTTTACCAC	55.0	39.1	58.6	57.3	635-804 bp	1.0	57.6
trnS-GCU-trnG-UCC_p3	CTTTCGTCCACTCAGCCATC	GAACGAATCACACTTTTACCACT	55.0	39.1	58.6	57.3	634-803 bp	1.0	57.6
trnS-GCU-trnG-UCC_p4	CTTTCGTCCACTCAGCCATC	AACGAATCACACTTTTACCACT	55.0	36.4	58.6	56.2	633-802 bp	1.0	56.7
trnS-GCU-trnG-UCC_p5	TTTCGTCCACTCAGCCATCT	ACGAATCACACTTTTACCACT	50.0	38.1	59.0	55.5	631-800 bp	1.0	55.5