Hotspot

rps16-trnQ-UUG

LSC · 6114-7239 · 1126 bp

Back to Lysimachia

Pi 0.0404

MarkerSeek score 79.80

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1126
variable_sites: 273
pi: 0.0404
unique_haplotype_count: 61
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	CGTTGCTTTCTACCACATCG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.2	58.1	1061-1801 bp	0.968254	83.8
rps16-trnQ-UUG_p2	GCACGTTGCTTTCTACCACA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	59.1	58.1	1064-1804 bp	1.0	82.9
rps16-trnQ-UUG_p3	CGTTGCTTTCTACCACATCG	AAATTTAAATGGGGCGTGGC	50.0	45.0	57.2	57.6	1118-1858 bp	0.968254	81.3
rps16-trnQ-UUG_p4	GCACGTTGCTTTCTACCACA	AAATTTAAATGGGGCGTGGC	50.0	45.0	59.1	57.6	1121-1861 bp	1.0	80.4
rps16-trnQ-UUG_p5	CGTTGCTTTCTACCACATCG	GGGTTTTGGTCCCGGTATTC	50.0	55.0	57.2	58.5	1082-1822 bp	0.968254	80.2