Hotspot

trnK-UUU-rps16

LSC · 4392-5022 · 631 bp

Back to Lysimachia

Pi 0.0605

MarkerSeek score 82.70

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 631
variable_sites: 209
pi: 0.0605
unique_haplotype_count: 60
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CAGGGATCTGGCTCTATCCA	AAAAGGGTATGTTGCTGCCA	55.0	45.0	58.0	58.0	1400-1680 bp	1.0	91.8
trnK-UUU-rps16_p2	ACAGGGATCTGGCTCTATCC	AAAAGGGTATGTTGCTGCCA	55.0	45.0	58.0	58.0	1401-1681 bp	1.0	91.8
trnK-UUU-rps16_p3	CAGGGATCTGGCTCTATCCA	CTATCCCACTGAGCCGTCTA	55.0	55.0	58.0	58.0	923-1226 bp	1.0	91.7
trnK-UUU-rps16_p4	ACAGGGATCTGGCTCTATCC	CTATCCCACTGAGCCGTCTA	55.0	55.0	58.0	58.0	924-1227 bp	1.0	91.7
trnK-UUU-rps16_p5	AAAAAGCCTTTTTCGCACCC	AAAAGGGTATGTTGCTGCCA	45.0	45.0	58.1	58.0	1532-1812 bp	0.984127	90.9