Hotspot

trnH-GUG-psbA

LSC · 99-525 · 427 bp

Back to Lysimachia

Pi 0.0476

MarkerSeek score 75.40

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 427
variable_sites: 124
pi: 0.0476
unique_haplotype_count: 57
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CTGCCTTAATCCACTTGGCT	CTGCGACTGCTGTTTTCTTG	50.0	50.0	57.9	58.0	848-1159 bp	1.0	90.7
trnH-GUG-psbA_p2	CTGCCTTAATCCACTTGGCT	TCAGTGCTATGCATGGTTCC	50.0	50.0	57.9	58.0	674-985 bp	0.984127	89.9
trnH-GUG-psbA_p3	ATAGTTATGGGCGAACGACG	CTGCGACTGCTGTTTTCTTG	50.0	50.0	57.9	58.0	903-1214 bp	0.52381	71.7
trnH-GUG-psbA_p4	ATAGTTATGGGCGAACGACG	TCAGTGCTATGCATGGTTCC	50.0	50.0	57.9	58.0	729-1040 bp	0.52381	71.6
trnH-GUG-psbA_p5	ATAGTTATGGGCGAACGACG	CAGTGCTATGCATGGTTCCT	50.0	50.0	57.9	58.0	728-1039 bp	0.52381	71.5