Hotspot

rrn5S-rrn4.5S

IRa · 135360-135582 · 223 bp

Back to Lysidice

Pi 0.0717

MarkerSeek score 84.00

Primer available yes

Label rrn5S-rrn4.5S

Feature Metadata

label_name: rrn5S-rrn4.5S
feature_type: igs
region: IRa
length_bp: 223
variable_sites: 16
pi: 0.0717
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn5S-rrn4.5S_p1	AGTATCGTCACCGCAGTAGA	CAGCTGAGGCATCCTAACAG	50.0	55.0	58.0	58.0	340 bp	1.0	83.1
rrn5S-rrn4.5S_p2	TATCGTCACCGCAGTAGAGT	CAGCTGAGGCATCCTAACAG	50.0	55.0	58.0	58.0	338 bp	1.0	83.1
rrn5S-rrn4.5S_p3	TATCGTCACCGCAGTAGAGT	GCTGAGGCATCCTAACAGAC	50.0	55.0	58.0	58.1	336 bp	1.0	83.1
rrn5S-rrn4.5S_p4	AGTATCGTCACCGCAGTAGA	GTCAAGTGGAAGTGCAGTGA	50.0	50.0	58.0	58.0	366 bp	1.0	82.9
rrn5S-rrn4.5S_p5	TATCGTCACCGCAGTAGAGT	GTCAAGTGGAAGTGCAGTGA	50.0	50.0	58.0	58.0	364 bp	1.0	82.9