Hotspot

trnR-ACG-rrn5S

IRa · 134966-135238 · 273 bp

Back to Lysidice

Pi 0
MarkerSeek score 56.70
Primer available yes
Label trnR-ACG-rrn5S

Feature Metadata

label_name
trnR-ACG-rrn5S
feature_type
igs
region
IRa
length_bp
273
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-ACG-rrn5S_p1 CCACGTGCTCTAATCCTCTG GGTGACGATACTGTAGGGGA 55.0 55.0 57.8 57.9 337-357 bp 1.0 81.8
trnR-ACG-rrn5S_p2 CCACGTGCTCTAATCCTCTG AATCCATCCCGAACTTGGTG 55.0 50.0 57.8 57.9 372-392 bp 1.0 81.2
trnR-ACG-rrn5S_p3 CCACGTGCTCTAATCCTCTG ACCAATCCATCCCGAACTTG 55.0 50.0 57.8 57.9 375-395 bp 1.0 81.2
trnR-ACG-rrn5S_p4 CCACGTGCTCTAATCCTCTG CAATCCATCCCGAACTTGGT 55.0 50.0 57.8 57.9 373-393 bp 1.0 81.2
trnR-ACG-rrn5S_p5 CCACGTGCTCTAATCCTCTG CACCAATCCATCCCGAACTT 55.0 50.0 57.8 57.9 376-396 bp 1.0 81.2