Hotspot

petA-psbJ

LSC · 65770-66624 · 855 bp

Back to Lysidice

Pi 0.0114

MarkerSeek score 69.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 855
variable_sites: 9
pi: 0.0114
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGTGTCCAAGGTCTCTTGTT	TCCGGATTAGGTTCATCCCT	50.0	50.0	57.8	57.5	930-990 bp	1.0	78.9
petA-psbJ_p2	CGTGTCCAAGGTCTCTTGTT	CCGGATTAGGTTCATCCCTG	50.0	55.0	57.8	57.4	929-989 bp	1.0	78.4
petA-psbJ_p3	CGTTACGTGTCCAAGGTCTC	TCCGGATTAGGTTCATCCCT	55.0	50.0	58.3	57.5	935-995 bp	1.0	78.4
petA-psbJ_p4	GATCCGTTACGTGTCCAAGG	TCCGGATTAGGTTCATCCCT	55.0	50.0	58.4	57.5	939-999 bp	1.0	77.9
petA-psbJ_p5	CGTTACGTGTCCAAGGTCTC	CCGGATTAGGTTCATCCCTG	55.0	55.0	58.3	57.4	934-994 bp	1.0	77.9