Hotspot

accD-psaI

LSC · 62056-62604 · 549 bp

Back to Lysidice

Pi 0.0171

MarkerSeek score 70.20

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 549
variable_sites: 9
pi: 0.0171
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCAATCGTACCGCGTAATCC	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.9	58.0	702-715 bp	1.0	82.3
accD-psaI_p2	CAATCGTACCGCGTAATCCT	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.9	58.0	701-714 bp	1.0	82.3
accD-psaI_p3	TCAATCGTACCGCGTAATCC	TGCCGGAAATACTAGACCCA	50.0	50.0	57.9	58.1	692-705 bp	1.0	81.5
accD-psaI_p4	CAATCGTACCGCGTAATCCT	TGCCGGAAATACTAGACCCA	50.0	50.0	57.9	58.1	691-704 bp	1.0	81.5
accD-psaI_p5	TCAATCGTACCGCGTAATCC	GCCGGAAATACTAGACCCAC	50.0	55.0	57.9	57.8	691-704 bp	1.0	80.7