Hotspot

trnL-UAA-trnF-GAA

LSC · 50898-51329 · 432 bp

Back to Lysidice

Pi 0.00928

MarkerSeek score 60.80

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 432
variable_sites: 4
pi: 0.00928
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	AAAGATAGAGTCCCGCTCCA	TCTACCAGCTGAGCTATCCC	50.0	55.0	57.8	58.0	563-564 bp	1.0	79.4
trnL-UAA-trnF-GAA_p2	AAAGATAGAGTCCCGCTCCA	TTTGAACTGGTGACACGAGG	50.0	50.0	57.8	58.1	599-600 bp	1.0	79.0
trnL-UAA-trnF-GAA_p3	AAAGATAGAGTCCCGCTCCA	GTGCCAGGAACCAGATTTGA	50.0	50.0	57.8	58.1	614-615 bp	1.0	78.6
trnL-UAA-trnF-GAA_p4	CCCGCTCCACATGTCAATAT	TCTACCAGCTGAGCTATCCC	50.0	55.0	57.7	58.0	552-553 bp	1.0	78.6
trnL-UAA-trnF-GAA_p5	AAAGATAGAGTCCCGCTCCA	TCAGTCCTCTGCTCTACCAG	50.0	55.0	57.8	57.9	575-576 bp	1.0	78.3