Hotspot

trnT-UGU-trnL-UAA

LSC · 49317-50218 · 902 bp

Back to Lysidice

Pi 0.0318

MarkerSeek score 74.30

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 902
variable_sites: 24
pi: 0.0318
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	55.0	50.0	57.9	58.1	849-960 bp	1.0	84.5
trnT-UGU-trnL-UAA_p2	TAGGGGAAAAGAAAAGCCGG	TAGCGTCTACCAATTTCGCC	50.0	50.0	57.8	58.1	906-1017 bp	1.0	83.8
trnT-UGU-trnL-UAA_p3	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	50.0	50.0	57.8	58.1	881-992 bp	1.0	83.6
trnT-UGU-trnL-UAA_p4	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	55.0	50.0	57.9	58.2	855-966 bp	1.0	83.1
trnT-UGU-trnL-UAA_p5	TAGGGGAAAAGAAAAGCCGG	AGTCCGTAGCGTCTACCAAT	50.0	50.0	57.8	58.2	912-1023 bp	1.0	82.4