Hotspot

psbZ-trnG-GCC

LSC · 38955-39543 · 589 bp

Back to Lysidice

Pi 0.0502

MarkerSeek score 87.10

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 589
variable_sites: 28
pi: 0.0502
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 87.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CTTCTCCTGATGGTTGGTCG	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.0	57.8	732-743 bp	1.0	84.9
psbZ-trnG-GCC_p2	TTGCTTCTCCTGATGGTTGG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	735-746 bp	1.0	83.4
psbZ-trnG-GCC_p3	TCCTGATGGTTGGTCGAGTA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	728-739 bp	1.0	83.3
psbZ-trnG-GCC_p4	GCTTCTCCTGATGGTTGGTC	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.3	57.8	733-744 bp	1.0	83.0
psbZ-trnG-GCC_p5	CTTCTCCTGATGGTTGGTCG	GGATAGCGGGAATTGAACCC	55.0	55.0	58.0	58.4	753-764 bp	1.0	82.9