Hotspot

trnC-GCA-petN

LSC · 29135-30236 · 1102 bp

Back to Lysidice

Pi 0.00764

MarkerSeek score 67.90

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 1102
variable_sites: 8
pi: 0.00764
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	1113-1158 bp	1.0	82.6
trnC-GCA-petN_p2	TTTATCGTTTTGGCGGCATG	AGCCCAAGCGAGACTTACTA	45.0	50.0	57.7	58.2	1166-1211 bp	1.0	80.9
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	TCTTCCCCATACTACGAGGG	50.0	55.0	58.1	57.6	1164-1209 bp	1.0	80.6
trnC-GCA-petN_p4	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	1131-1176 bp	1.0	80.3
trnC-GCA-petN_p5	TTTATCGTTTTGGCGGCATG	TCTTCCCCATACTACGAGGG	45.0	55.0	57.7	57.6	1217-1262 bp	1.0	78.9