Hotspot

atpH-atpI

LSC · 14359-15667 · 1309 bp

Back to Lysidice

Pi 0.0261

MarkerSeek score 63.80

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1309
variable_sites: 25
pi: 0.0261
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.73
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AATAGAAGCAAGCCCGACAG	TTTGCAACTTTAGCTGCTGC	50.0	45.0	58.0	57.9	1081-1429 bp	1.0	84.2
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	TTTGCAACTTTAGCTGCTGC	45.0	45.0	58.0	57.9	1047-1395 bp	1.0	84.2
atpH-atpI_p3	TGGTCCAATAGAAGCAAGCC	TTTGCAACTTTAGCTGCTGC	50.0	45.0	57.9	57.9	1087-1435 bp	1.0	83.4
atpH-atpI_p4	AATAACGGAAGCGGCAGAAA	TTTGCAACTTTAGCTGCTGC	45.0	45.0	58.2	57.9	1048-1396 bp	1.0	83.1
atpH-atpI_p5	AATAGAAGCAAGCCCGACAG	GCTGCTGCTTATATAGGCGA	50.0	50.0	58.0	57.6	1069-1417 bp	1.0	82.4