Hotspot

trnS-GCU-trnG-UCC

LSC · 8368-9170 · 803 bp

Back to Lysidice

Pi 0.0129

MarkerSeek score 69.10

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 803
variable_sites: 10
pi: 0.0129
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	ATGAATCAAACGAGGGACCC	50.0	50.0	58.0	57.6	916-951 bp	1.0	79.6
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	ATGAATCAAACGAGGGACCC	50.0	50.0	58.0	57.6	915-950 bp	1.0	79.6
trnS-GCU-trnG-UCC_p3	CAGATTAGCAATCCGACGCT	ATGAATCAAACGAGGGACCC	50.0	50.0	58.1	57.6	924-959 bp	1.0	78.5
trnS-GCU-trnG-UCC_p4	ACGGAAAGAGAGGGATTCGA	ATGAATCAAACGAGGGACCC	50.0	50.0	58.2	57.6	970-1005 bp	1.0	78.3
trnS-GCU-trnG-UCC_p5	AGCAATCCGACGCTTTAGTC	ATGAATCAAACGAGGGACCC	50.0	50.0	58.4	57.6	918-953 bp	1.0	76.8