Hotspot

ycf1-chlN

SSC · 130124-130598 · 475 bp

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Pi 0.0691

MarkerSeek score 82.30

Primer available yes

Label ycf1-chlN

Feature Metadata

label_name: ycf1-chlN
feature_type: igs
region: SSC
length_bp: 475
variable_sites: 100
pi: 0.0691
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-chlN_p1	TTCCACCGATCTTCCCTTCT	GAGATACCAGGCTGCTGAAC	50.0	55.0	58.0	58.1	941-969 bp	0.875	84.4
ycf1-chlN_p2	TTCCACCGATCTTCCCTTCT	CATTCTTGAGTCGTACGGCA	50.0	50.0	58.0	58.0	1251-1279 bp	0.875	83.8
ycf1-chlN_p3	ACAAAGCCAATGTACAGGGG	CATTCTTGAGTCGTACGGCA	50.0	50.0	58.1	58.0	1847-1870 bp	0.5	65.4
ycf1-chlN_p4	TTCCACCGATCTTCCCTTCT	GGCTAAGATTTGGGAGAGCC	50.0	55.0	58.0	58.0	1110-1125 bp	0.375	59.8
ycf1-chlN_p5	ACAAAGCCAATGTACAGGGG	GGCTAAGATTTGGGAGAGCC	50.0	55.0	58.1	58.0	1706 bp	0.125	44.2