Hotspot

trnV-UAC-trnM-CAU

LSC · 51624-51845 · 222 bp

Back to Lygodium

Pi 0.0613

MarkerSeek score 82.80

Primer available yes

Label trnV-UAC-trnM-CAU

Feature Metadata

label_name: trnV-UAC-trnM-CAU
feature_type: igs
region: LSC
length_bp: 222
variable_sites: 42
pi: 0.0613
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-UAC-trnM-CAU_p1	TCTACCGCTGAGCTATAGCC	GCGATACTCTAACCGCTGAG	55.0	55.0	58.5	58.0	247-274 bp	1.0	88.0
trnV-UAC-trnM-CAU_p2	CGTGTAAACGAGGTGCTCTA	GCGATACTCTAACCGCTGAG	50.0	55.0	57.4	58.0	263-290 bp	1.0	86.7
trnV-UAC-trnM-CAU_p3	TCTACCGCTGAGCTATAGCC	TTCGAACCAATGACTCTCGC	55.0	50.0	58.5	58.3	276-303 bp	1.0	86.0
trnV-UAC-trnM-CAU_p4	CGTGTAAACGAGGTGCTCTA	TTCGAACCAATGACTCTCGC	50.0	50.0	57.4	58.3	292-319 bp	1.0	84.7
trnV-UAC-trnM-CAU_p5	TCCTTTCAAAAAGCATCGGC	GCGATACTCTAACCGCTGAG	45.0	55.0	57.3	58.0	311-313 bp	0.875	77.4