Hotspot

trnL-CAA-trnF-GAA

LSC · 48078-48493 · 416 bp

Back to Lygodium

Pi 0.0696
MarkerSeek score 86.50
Primer available yes
Label trnL-CAA-trnF-GAA

Feature Metadata

label_name
trnL-CAA-trnF-GAA
feature_type
igs
region
LSC
length_bp
416
variable_sites
90
pi
0.0696
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
86.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-CAA-trnF-GAA_p1 GGGTTCGAGTCCCTCTATCC TTCGAACTGATGACACGAGG 60.0 50.0 58.7 57.7 493-501 bp 1.0 83.1
trnL-CAA-trnF-GAA_p2 GGTTCGAGTCCCTCTATCCC TTCGAACTGATGACACGAGG 60.0 50.0 58.7 57.7 492-500 bp 1.0 83.1
trnL-CAA-trnF-GAA_p3 GTTCGAGTCCCTCTATCCCC TTCGAACTGATGACACGAGG 60.0 50.0 58.7 57.7 491-499 bp 1.0 83.1
trnL-CAA-trnF-GAA_p4 GGTTCGAGTCCCTCTATCCC TCGAACTGATGACACGAGGA 60.0 50.0 58.7 58.5 491-499 bp 1.0 82.1
trnL-CAA-trnF-GAA_p5 GTTCGAGTCCCTCTATCCCC TCGAACTGATGACACGAGGA 60.0 50.0 58.7 58.5 490-498 bp 1.0 82.1