Hotspot

psbD-trnT-GGU

LSC · 35616-37109 · 1494 bp

Back to Lygodium

Pi 0.0615

MarkerSeek score 85.00

Primer available yes

Label psbD-trnT-GGU

Feature Metadata

label_name: psbD-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1494
variable_sites: 283
pi: 0.0615
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 85.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnT-GGU_p1	CAACCGACAAAGACGAAACG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.0	58.0	2148-2225 bp	1.0	91.7
psbD-trnT-GGU_p2	CGAAATGTGTTTGCCCCATC	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.0	58.0	2751-2828 bp	1.0	91.7
psbD-trnT-GGU_p3	CAACCGACAAAGACGAAACG	ATGACCCTTAGTTGCTCCCT	50.0	50.0	58.0	58.0	1911-1974 bp	0.875	86.3
psbD-trnT-GGU_p4	GAGGATTTCCCAATGGCGAT	ATGACCCTTAGTTGCTCCCT	50.0	50.0	58.0	58.0	1839-1902 bp	0.875	86.3
psbD-trnT-GGU_p5	CGAAATGTGTTTGCCCCATC	ATGACCCTTAGTTGCTCCCT	50.0	50.0	58.0	58.0	2514-2577 bp	0.875	84.8