Hotspot

trnE-UUC-trnG-GCC

LSC · 30560-31259 · 700 bp

Back to Lygodium

Pi 0.0721
MarkerSeek score 84.90
Primer available yes
Label trnE-UUC-trnG-GCC

Feature Metadata

label_name
trnE-UUC-trnG-GCC
feature_type
igs
region
LSC
length_bp
700
variable_sites
151
pi
0.0721
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.95
markerseek_score
84.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnG-GCC_p1 CCTAGGCCTCTAGACGATGG GCGCGGATATAGTTGAATGG 60.0 50.0 58.5 56.8 759-797 bp 1.0 79.0
trnE-UUC-trnG-GCC_p2 CTAGGCCTCTAGACGATGGG GCGCGGATATAGTTGAATGG 60.0 50.0 58.5 56.8 758-796 bp 1.0 79.0
trnE-UUC-trnG-GCC_p3 CCTAGGCCTCTAGACGATGG GTTCGATTCCCGCTATCCG 60.0 57.9 58.5 58.2 707-745 bp 1.0 78.8
trnE-UUC-trnG-GCC_p4 CTAGGCCTCTAGACGATGGG GTTCGATTCCCGCTATCCG 60.0 57.9 58.5 58.2 706-744 bp 1.0 78.8
trnE-UUC-trnG-GCC_p5 CTAGGCCTCTAGACGATGGG GGTTCGATTCCCGCTATCC 60.0 57.9 58.5 57.8 707-745 bp 1.0 78.6