Hotspot

matK-rps16

LSC · 3214-4432 · 1219 bp

Back to Lygodium

Pi 0.0749

MarkerSeek score 83.30

Primer available yes

Label matK-rps16

Feature Metadata

label_name: matK-rps16
feature_type: igs
region: LSC
length_bp: 1219
variable_sites: 255
pi: 0.0749
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 83.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-rps16_p1	CTCGGACTTGTCGACGAAAT	TGACTATCCTGATACGCCGT	50.0	50.0	58.0	58.0	1877-1991 bp	0.625	76.6
matK-rps16_p2	AGAACCGCTCCCCAAATTTT	TGACTATCCTGATACGCCGT	45.0	50.0	58.0	58.0	1642-1756 bp	0.375	65.6
matK-rps16_p3	AGAACCGCTCCCCAAATTTT	CCATGAACGAGGCAATACCA	45.0	50.0	58.0	58.0	1922-2037 bp	0.25	57.8
matK-rps16_p4	AAGAACCGCTCCCCAAATTT	TGACTATCCTGATACGCCGT	45.0	50.0	58.0	58.0	1757 bp	0.125	44.7
matK-rps16_p5	AAGAACCGCTCCCCAAATTT	CCATGAACGAGGCAATACCA	45.0	50.0	58.0	58.0	2038 bp	0.125	44.6