Hotspot

ndhA

SSC · 123014-125236 · 2223 bp

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Pi 0.00336

MarkerSeek score 47.10

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2223
variable_sites: 31
pi: 0.00336
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ATGAACCCGGTCACCATAGG	GTCCACCCGGTTTCATCAAT	55.0	50.0	59.2	58.2	2457-2464 bp	1.0	70.4
ndhA_p2	CCGGTCACCATAGGGAACAT	GTCCACCCGGTTTCATCAAT	55.0	50.0	59.2	58.2	2451-2458 bp	1.0	70.4
ndhA_p3	ATTTATGAACCCGGTCACCA	GTCCACCCGGTTTCATCAAT	45.0	50.0	56.8	58.2	2461-2468 bp	1.0	70.2
ndhA_p4	TTTATGAACCCGGTCACCAT	GTCCACCCGGTTTCATCAAT	45.0	50.0	56.8	58.2	2460-2467 bp	1.0	70.2
ndhA_p5	AATTTATGAACCCGGTCACCA	GTCCACCCGGTTTCATCAAT	42.9	50.0	57.6	58.2	2462-2469 bp	1.0	68.2