Hotspot

clpP

LSC · 72535-74592 · 2058 bp

Back to Lycoris

Pi 0.00302

MarkerSeek score 46.50

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2058
variable_sites: 20
pi: 0.00302
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	ATGGTTTGAAACGGAATCGC	AACTCATTCTTACGTTTCCA	45.0	35.0	57.4	52.5	2143-2155 bp	1.0	41.2
clpP_p2	ATGGTTTGAAACGGAATCGC	AAACTCATTCTTACGTTTCCA	45.0	33.3	57.4	53.4	2144-2156 bp	1.0	41.2
clpP_p3	GTTTGAAACGGAATCGCGTA	AACTCATTCTTACGTTTCCA	45.0	35.0	57.1	52.5	2140-2152 bp	1.0	41.2
clpP_p4	ATGGTTTGAAACGGAATCGC	ACTCATTCTTACGTTTCCAT	45.0	35.0	57.4	52.2	2142-2154 bp	1.0	41.2
clpP_p5	GTTTGAAACGGAATCGCGTA	AAACTCATTCTTACGTTTCCA	45.0	33.3	57.1	53.4	2141-2153 bp	1.0	41.2