Hotspot

cemA

LSC · 63709-64434 · 726 bp

Back to Lycoris

Pi 0.00232

MarkerSeek score 45.40

Primer available yes

Label cemA

Feature Metadata

label_name: cemA
feature_type: gene
region: LSC
length_bp: 726
variable_sites: 5
pi: 0.00232
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA_p1	AGAACTCGCGCTTTAGAGAA	ACGCTTTCTTTATACGCGAAG	45.0	42.9	57.0	57.1	823-830 bp	1.0	57.4
cemA_p2	CCTTGTTATAGAACTCGCGCT	ACGCTTTCTTTATACGCGAAG	47.6	42.9	58.2	57.1	832-839 bp	1.0	55.9
cemA_p3	AGAACTCGCGCTTTAGAGAA	AACGCTTTCTTTATACGCGA	45.0	40.0	57.0	55.9	824-831 bp	1.0	55.9
cemA_p4	AGAACTCGCGCTTTAGAGAA	ACGCTTTCTTTATACGCGAA	45.0	40.0	57.0	55.9	823-830 bp	1.0	55.9
cemA_p5	AGAACTCGCGCTTTAGAGAA	CGCTTTCTTTATACGCGAAGA	45.0	42.9	57.0	56.8	822-829 bp	1.0	55.2