Hotspot

rpl32-trnL-UAG

SSC · 116530-117349 · 820 bp

Back to Lycoris

Pi 0.00943
MarkerSeek score 44.60
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
820
variable_sites
25
pi
0.00943
unique_haplotype_count
13
primer_available
yes
alignment_reliability
1.00
markerseek_score
44.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TGTGCGACAAACAAGTAAGA TTTTTGAGTATGCCGCCACT 40.0 45.0 55.6 58.1 918-928 bp 1.0 62.5
rpl32-trnL-UAG_p2 TGTGCGACAAACAAGTAAGA TCTTTTTGAGTATGCCGCCA 40.0 45.0 55.6 57.8 920-930 bp 1.0 61.7
rpl32-trnL-UAG_p3 TGTGCGACAAACAAGTAAGA CTTTTTGAGTATGCCGCCAC 40.0 50.0 55.6 57.7 919-929 bp 1.0 61.2
rpl32-trnL-UAG_p4 TGTGCGACAAACAAGTAAGAA TTTTTGAGTATGCCGCCACT 38.1 45.0 56.3 58.1 918-928 bp 1.0 60.7
rpl32-trnL-UAG_p5 TTGTGCGACAAACAAGTAAGA TTTTTGAGTATGCCGCCACT 38.1 45.0 56.3 58.1 919-929 bp 1.0 60.7