Hotspot

ndhF-rpl32

SSC · 115421-116355 · 935 bp

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Pi 0.00585
MarkerSeek score 52.60
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
935
variable_sites
23
pi
0.00585
unique_haplotype_count
11
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TGTTGCTGTCGGAATAAGCA AGAAAAAGCTTTTATGGCAGC 45.0 38.1 57.8 55.6 1001-1155 bp 1.0 57.5
ndhF-rpl32_p2 TGTTGCTGTCGGAATAAGCA AAGAAAAAGCTTTTATGGCAGC 45.0 36.4 57.8 56.4 1002-1156 bp 1.0 55.4
ndhF-rpl32_p3 TGTTGCTGTCGGAATAAGCA TGGCAGCTAAATATCCCTTT 45.0 40.0 57.8 54.4 987-1141 bp 1.0 55.1
ndhF-rpl32_p4 TGTTGCTGTCGGAATAAGCA TGGCAGCTAAATATCCCTTTT 45.0 38.1 57.8 55.2 987-1141 bp 1.0 54.1
ndhF-rpl32_p5 TGTTGCTGTCGGAATAAGCA TGGCAGCTAAATATCCCTTTTTC 45.0 39.1 57.8 57.1 987-1141 bp 1.0 53.5