Hotspot

ndhC-trnV-UAC

LSC · 52400-53989 · 1590 bp

Back to Lycoris

Pi 0.00362

MarkerSeek score 48.00

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1590
variable_sites: 17
pi: 0.00362
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGGGCTAAAACTCCGGAAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	1713-1733 bp	1.0	78.6
ndhC-trnV-UAC_p2	GGGGCTAAAACTCCGGAAAT	AGCCCAATGCAACAAAATGC	50.0	45.0	57.9	58.5	1795-1815 bp	1.0	76.3
ndhC-trnV-UAC_p3	TCCTTCACTAATCGGGGCTA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.5	57.8	1726-1746 bp	1.0	76.0
ndhC-trnV-UAC_p4	GGGGCTAAAACTCCGGAAAT	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.9	57.5	1712-1732 bp	1.0	75.8
ndhC-trnV-UAC_p5	CTGGTCCTTCACTAATCGGG	AGAAGGTCTACGGTTCGAGT	55.0	50.0	57.4	57.8	1730-1750 bp	0.904762	71.0