Hotspot

trnT-UGU-trnL-UAA

LSC · 48052-49022 · 971 bp

Back to Lycoris

Pi 0.00801

MarkerSeek score 52.10

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 971
variable_sites: 61
pi: 0.00801
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	GGAAGTTCCCACTTAGCAGG	55.0	55.0	57.9	57.9	1040-1102 bp	1.0	86.5
trnT-UGU-trnL-UAA_p2	GCGATGCTCTAACCTCTGAG	ATCCAATCAAGTCCGTAGCG	55.0	50.0	57.9	57.8	1002-1064 bp	1.0	85.8
trnT-UGU-trnL-UAA_p3	GGAATCGAACCGATGACCAT	GGAAGTTCCCACTTAGCAGG	50.0	55.0	57.8	57.9	1072-1134 bp	1.0	85.5
trnT-UGU-trnL-UAA_p4	GGAATCGAACCGATGACCAT	ATCCAATCAAGTCCGTAGCG	50.0	50.0	57.8	57.8	1034-1096 bp	1.0	84.8
trnT-UGU-trnL-UAA_p5	CTAACCTCTGAGCTAAGCGG	GGAAGTTCCCACTTAGCAGG	55.0	55.0	57.5	57.9	1032-1094 bp	1.0	83.6