Hotspot

ycf3-trnS-GGA

LSC · 46043-46660 · 618 bp

Back to Lycoris

Pi 0.00106

MarkerSeek score 47.30

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 618
variable_sites: 6
pi: 0.00106
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TTCTCCTGAAGTTGTCGGAA	ACGGAAAGAGAGGGATTCGA	45.0	50.0	56.4	58.2	766-796 bp	1.0	68.5
ycf3-trnS-GGA_p2	TTTCTCCTGAAGTTGTCGGA	ACGGAAAGAGAGGGATTCGA	45.0	50.0	56.4	58.2	767-797 bp	1.0	68.5
ycf3-trnS-GGA_p3	TCTCCTGAAGTTGTCGGAAT	ACGGAAAGAGAGGGATTCGA	45.0	50.0	56.2	58.2	765-795 bp	1.0	66.6
ycf3-trnS-GGA_p4	TTTCTCCTGAAGTTGTCGGAA	ACGGAAAGAGAGGGATTCGA	42.9	50.0	57.2	58.2	767-797 bp	1.0	66.5
ycf3-trnS-GGA_p5	TTTTCTCCTGAAGTTGTCGGA	ACGGAAAGAGAGGGATTCGA	42.9	50.0	57.2	58.2	768-798 bp	1.0	66.5