Hotspot

psaA-ycf3

LSC · 43380-44052 · 673 bp

Back to Lycoris

Pi 0.00669

MarkerSeek score 45.40

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 673
variable_sites: 21
pi: 0.00669
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CCCATTCCTCGAAAGACGTT	ACCAAGCTGCTGAGTATTGG	50.0	50.0	57.9	57.9	844-867 bp	1.0	81.6
psaA-ycf3_p2	CCCATTCCTCGAAAGACGTT	TTGAAGATCACGAGGCGTTT	50.0	45.0	57.9	57.8	773-796 bp	1.0	81.5
psaA-ycf3_p3	CCCATTCCTCGAAAGACGTT	TTGGTTGAAGATCACGAGGC	50.0	50.0	57.9	58.2	777-800 bp	1.0	81.3
psaA-ycf3_p4	CCCATTCCTCGAAAGACGTT	TGAAGATCACGAGGCGTTTC	50.0	50.0	57.9	58.3	772-795 bp	1.0	80.5
psaA-ycf3_p5	CCCATTCCTCGAAAGACGTT	TCACGAGGCGTTTCGAATAA	50.0	45.0	57.9	57.6	766-789 bp	1.0	80.0