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psbZ-trnG-GCC

LSC · 37633-37963 · 331 bp

Back to Lycoris

Pi 0.0186

MarkerSeek score 49.80

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 331
variable_sites: 15
pi: 0.0186
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TGCTTCTTCTGATGGTTGGT	GATCGAACCCGCATCTTCTC	45.0	55.0	57.0	58.5	474-476 bp	1.0	71.1
psbZ-trnG-GCC_p2	TGCTTCTTCTGATGGTTGGT	GAACCCGCATCTTCTCCTTG	45.0	55.0	57.0	58.6	470-472 bp	1.0	70.2
psbZ-trnG-GCC_p3	GCTTCTTCTGATGGTTGGTCA	GATCGAACCCGCATCTTCTC	47.6	55.0	58.2	58.5	473-475 bp	1.0	69.1
psbZ-trnG-GCC_p4	TGCTTCTTCTGATGGTTGGTC	GATCGAACCCGCATCTTCTC	47.6	55.0	58.2	58.5	474-476 bp	1.0	69.1
psbZ-trnG-GCC_p5	TTGCTTCTTCTGATGGTTGGT	GATCGAACCCGCATCTTCTC	42.9	55.0	57.8	58.5	475-477 bp	1.0	69.0