Hotspot

ycf1-trnD-GUC

LSC · 30956-32003 · 1048 bp

Back to Lycoris

Pi 0.00341

MarkerSeek score 48.50

Primer available yes

Label ycf1-trnD-GUC

Feature Metadata

label_name: ycf1-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1048
variable_sites: 15
pi: 0.00341
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-trnD-GUC_p1	AGAATGAACAGTGCAGTAGC	GTATGCTATACACCTCGCCG	45.0	55.0	55.8	58.0	1151-1170 bp	1.0	64.3
ycf1-trnD-GUC_p2	AGAATGAACAGTGCAGTAGC	AGTTCAATCGGTCAGAGCAC	45.0	50.0	55.8	57.9	1124-1143 bp	1.0	63.8
ycf1-trnD-GUC_p3	AGGAACTAGAATGAACAGTGCA	GTATGCTATACACCTCGCCG	40.9	55.0	57.4	58.0	1158-1177 bp	1.0	61.1
ycf1-trnD-GUC_p4	AGGAACTAGAATGAACAGTGCA	AGTTCAATCGGTCAGAGCAC	40.9	50.0	57.4	57.9	1131-1150 bp	1.0	60.6
ycf1-trnD-GUC_p5	GGAACTAGAATGAACAGTGCAG	GTATGCTATACACCTCGCCG	45.5	55.0	57.1	58.0	1157-1176 bp	1.0	58.6