Hotspot

trnS-GCU-trnG-UCC

LSC · 8604-9808 · 1205 bp

Back to Lycoris

Pi 0.00842

MarkerSeek score 56.60

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 1205
variable_sites: 49
pi: 0.00842
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ATTAGCAATCCGCCGCTTTA	CGAACGAATCACACTTTTACCA	45.0	40.9	58.0	57.3	1266-1297 bp	1.0	63.2
trnS-GCU-trnG-UCC_p2	ATTAGCAATCCGCCGCTTTA	TCGAACGAATCACACTTTTACC	45.0	40.9	58.0	57.0	1267-1298 bp	1.0	61.2
trnS-GCU-trnG-UCC_p3	CGGAGAGAGAGGGATTCGAA	CGAACGAATCACACTTTTACCA	55.0	40.9	58.3	57.3	1314-1345 bp	1.0	60.8
trnS-GCU-trnG-UCC_p4	GCTTTAGTCCACTCAGCCAT	CGAACGAATCACACTTTTACCA	50.0	40.9	57.6	57.3	1252-1283 bp	1.0	60.3
trnS-GCU-trnG-UCC_p5	AGTCCACTCAGCCATCTCTC	CGAACGAATCACACTTTTACCA	55.0	40.9	58.5	57.3	1247-1278 bp	1.0	59.5