Hotspot

rps19

LSC · 82868-83146 · 279 bp

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Pi 0.00717

MarkerSeek score 57.10

Primer available yes

Label rps19

Feature Metadata

label_name: rps19
feature_type: gene
region: LSC
length_bp: 279
variable_sites: 3
pi: 0.00717
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19_p1	CCTAAAGCGCGTACTTCTGT	GATTGTTCGTCGCCGTAGTA	50.0	50.0	58.0	57.8	473 bp	1.0	79.0
rps19_p2	CCTAAAGCGCGTACTTCTGT	TTTGATTGTTCGTCGCCGTA	50.0	45.0	58.0	58.2	476 bp	1.0	78.7
rps19_p3	CCTAAAGCGCGTACTTCTGT	AATTTGATTGTTCGTCGCCG	50.0	45.0	58.0	57.8	478 bp	1.0	78.5
rps19_p4	TATATGTCGGCCTAAAGCGC	GATTGTTCGTCGCCGTAGTA	50.0	50.0	57.9	57.8	483 bp	1.0	78.5
rps19_p5	TATATGTCGGCCTAAAGCGC	TTTGATTGTTCGTCGCCGTA	50.0	45.0	57.9	58.2	486 bp	1.0	78.1