Hotspot

rpl16

LSC · 80252-81532 · 1281 bp

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Pi 0.00156

MarkerSeek score 48.10

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1281
variable_sites: 3
pi: 0.00156
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	CCAAGACTCATTTCTGTCCGT	AGGAAAAAGGGGAAACTCCT	47.6	45.0	58.0	56.2	1423 bp	1.0	57.6
rpl16_p2	CCAAGACTCATTTCTGTCCGT	AAGGAAAAAGGGGAAACTCCT	47.6	42.9	58.0	57.0	1424 bp	1.0	55.9
rpl16_p3	CCAAGACTCATTTCTGTCCGT	AGGAAAAAGGGGAAACTCCTT	47.6	42.9	58.0	57.0	1423 bp	1.0	55.9
rpl16_p4	CCCCAAGACTCATTTCTGTCC	AGGAAAAAGGGGAAACTCCT	52.4	45.0	58.3	56.2	1425 bp	1.0	55.7
rpl16_p5	CCAAGACTCATTTCTGTCCGT	AGGAAAAAGGGGAAACTCCTTC	47.6	45.5	58.0	58.2	1423 bp	1.0	54.6