Hotspot

ndhE

SSC · 115851-116156 · 306 bp

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Pi 0.00654

MarkerSeek score 32.60

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: SSC
length_bp: 306
variable_sites: 3
pi: 0.00654
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 32.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	TCAATTTTCCACTGATACAATGA	ACCAATCTATTTCCTTGTTTCA	30.4	31.8	54.3	53.6	432 bp	1.0	40.6
ndhE_p2	TCAATTTTCCACTGATACAATGAA	ACCAATCTATTTCCTTGTTTCA	29.2	31.8	55.0	53.6	432 bp	1.0	40.6
ndhE_p3	TTCAATTTTCCACTGATACAATGA	ACCAATCTATTTCCTTGTTTCA	29.2	31.8	55.0	53.6	433 bp	1.0	40.6
ndhE_p4	TCAATTTTCCACTGATACAATGA	ACCAATCTATTTCCTTGTTTCAT	30.4	30.4	54.3	54.2	432 bp	1.0	40.6
ndhE_p5	CAATTTTCCACTGATACAATGA	ACCAATCTATTTCCTTGTTTCA	31.8	31.8	52.7	53.6	431 bp	1.0	40.6