Hotspot

rps15-ycf1

SSC · 121523-121964 · 442 bp

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Pi 0.00452
MarkerSeek score 34.80
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
442
variable_sites
3
pi
0.00452
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
34.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 ACTTGAAATTCAACAGACCCTCT CCGCGATTGAAATTCTGTGA 39.1 45.0 57.9 56.8 543-551 bp 1.0 46.6
rps15-ycf1_p2 ACTTGAAATTCAACAGACCCTCT TCCGCGATTGAAATTCTGTG 39.1 45.0 57.9 56.8 544-552 bp 1.0 46.6
rps15-ycf1_p3 ACAGACCCTCTATTTTCTTCGT CCGCGATTGAAATTCTGTGA 40.9 45.0 56.8 56.8 531-539 bp 1.0 45.7
rps15-ycf1_p4 ACAGACCCTCTATTTTCTTCGT TCCGCGATTGAAATTCTGTG 40.9 45.0 56.8 56.8 532-540 bp 1.0 45.7
rps15-ycf1_p5 ACTTGAAATTCAACAGACCCTCT TCCGCGATTGAAATTCTGTGA 39.1 42.9 57.9 58.3 544-552 bp 1.0 45.6