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rps8-rpl14

LSC · 79566-79755 · 190 bp

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Pi 0.00353

MarkerSeek score 57.60

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 190
variable_sites: 1
pi: 0.00353
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	AGATGCTATGCGAACAACTCT	GGAACTCGAATTTTTGGGGC	42.9	50.0	57.5	57.6	356-361 bp	1.0	66.2
rps8-rpl14_p2	AGATGCTATGCGAACAACTC	GGAACTCGAATTTTTGGGGC	45.0	50.0	55.9	57.6	356-361 bp	1.0	62.0
rps8-rpl14_p3	GATGCTATGCGAACAACTCT	GGAACTCGAATTTTTGGGGC	45.0	50.0	55.9	57.6	355-360 bp	1.0	62.0
rps8-rpl14_p4	AGATGCTATGCGAACAACTCT	CCCCGCGAATTGAGACAATT	42.9	50.0	57.5	58.9	332-337 bp	1.0	61.9
rps8-rpl14_p5	AGATGCTATGCGAACAACTCTT	GGAACTCGAATTTTTGGGGC	40.9	50.0	58.1	57.6	356-361 bp	1.0	61.4