Hotspot

ycf3-trnS-GGA

LSC · 43890-44521 · 632 bp

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Pi 0.00105

MarkerSeek score 61.20

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 632
variable_sites: 1
pi: 0.00105
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TGCCTCCTTTTCTCCTGAAG	CCCTCGGTAAACAAAAGCCT	50.0	50.0	57.1	58.1	795-804 bp	1.0	73.4
ycf3-trnS-GGA_p2	GCCTCCTTTTCTCCTGAAGT	CCCTCGGTAAACAAAAGCCT	50.0	50.0	57.1	58.1	794-803 bp	1.0	73.4
ycf3-trnS-GGA_p3	TGCCTCCTTTTCTCCTGAAG	AAGTGAAGGTGCGGAAAGAG	50.0	50.0	57.1	58.1	826-835 bp	1.0	73.2
ycf3-trnS-GGA_p4	GCCTCCTTTTCTCCTGAAGT	AAGTGAAGGTGCGGAAAGAG	50.0	50.0	57.1	58.1	825-834 bp	1.0	73.2
ycf3-trnS-GGA_p5	TGCCTCCTTTTCTCCTGAAG	TTCCAATGCTACGCCTTGAA	50.0	45.0	57.1	57.8	766-775 bp	1.0	72.7