Hotspot

rps2-rpoC2

LSC · 15959-16172 · 214 bp

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Pi 0.00935

MarkerSeek score 58.50

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 214
variable_sites: 3
pi: 0.00935
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	CCTCCAGCATCTCTTCCAAG	TTCGAGGGGGAAATGAGAGA	55.0	50.0	57.7	57.8	377-379 bp	1.0	76.0
rps2-rpoC2_p2	CCTCCAGCATCTCTTCCAAG	TCGAGGGGGAAATGAGAGAT	55.0	50.0	57.7	57.5	376-378 bp	1.0	74.2
rps2-rpoC2_p3	CATGGCCAAAATGAACTCCC	TTCGAGGGGGAAATGAGAGA	50.0	50.0	57.3	57.8	398-400 bp	1.0	73.2
rps2-rpoC2_p4	CCATGGCCAAAATGAACTCC	TTCGAGGGGGAAATGAGAGA	50.0	50.0	57.3	57.8	399-401 bp	1.0	73.2
rps2-rpoC2_p5	ATGGCCAAAATGAACTCCCG	TTCGAGGGGGAAATGAGAGA	50.0	50.0	58.8	57.8	397-399 bp	1.0	72.1