Hotspot

trnR-UCU-atpA

LSC · 10125-10232 · 108 bp

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Pi 0.0123

MarkerSeek score 61.40

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 108
variable_sites: 2
pi: 0.0123
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	AAAAATCGGAATGAACGGCG	CCGGTTTCTACTTCAGGAGC	45.0	55.0	57.7	58.0	224-226 bp	1.0	78.9
trnR-UCU-atpA_p2	GAACGGCGTCCATTGTCTAA	CCGGTTTCTACTTCAGGAGC	50.0	55.0	58.3	58.0	212-214 bp	1.0	78.8
trnR-UCU-atpA_p3	ACGGCGTCCATTGTCTAATG	CCGGTTTCTACTTCAGGAGC	50.0	55.0	58.3	58.0	210-212 bp	1.0	78.3
trnR-UCU-atpA_p4	AAAAATCGGAATGAACGGCG	TTCAGGAACAAATGGACCGG	45.0	50.0	57.7	58.1	240-242 bp	1.0	78.2
trnR-UCU-atpA_p5	GAACGGCGTCCATTGTCTAA	TTCAGGAACAAATGGACCGG	50.0	50.0	58.3	58.1	228-230 bp	1.0	78.0