Hotspot

ycf66

Genome · 98838-99927 · 1090 bp

Back to Lycopodium

Pi 0.0112

MarkerSeek score 53.30

Primer available yes

Label ycf66

Feature Metadata

label_name: ycf66
feature_type: gene
region: Genome
length_bp: 1090
variable_sites: 12
pi: 0.0112
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 53.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf66_p1	TGTAGTCTGGGGAAGGAGTG	TGAAATGGTAGACACGCGAG	55.0	50.0	58.1	58.0	1886-1901 bp	1.0	81.3
ycf66_p2	CTCCACTTGTAGTCTGGGGA	TGAAATGGTAGACACGCGAG	55.0	50.0	58.1	58.0	1893-1908 bp	1.0	81.2
ycf66_p3	TCCTCCACTTGTAGTCTGGG	TGAAATGGTAGACACGCGAG	55.0	50.0	58.1	58.0	1895-1910 bp	1.0	81.2
ycf66_p4	TATTGCTTGGGCTGCTTTGA	TGAAATGGTAGACACGCGAG	45.0	50.0	58.1	58.0	1934-1949 bp	1.0	81.1
ycf66_p5	TGTAGTCTGGGGAAGGAGTG	AAAATCTCGTGCTGCAAAGC	55.0	45.0	58.1	57.9	1862-1877 bp	1.0	80.9