Hotspot

rpoC1

Genome · 91747-94485 · 2739 bp

Back to Lycopodium

Pi 0.00438

MarkerSeek score 51.70

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: Genome
length_bp: 2739
variable_sites: 12
pi: 0.00438
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	TGTCGTTATAGCTGATAGTATTCCA	ACGATGTTTAGCCCTGAATT	36.0	40.0	57.2	55.0	2864-2865 bp	1.0	40.4
rpoC1_p2	TGTCGTTATAGCTGATAGTATTCCA	ACGATGTTTAGCCCTGAATTT	36.0	38.1	57.2	55.8	2864-2865 bp	1.0	40.4
rpoC1_p3	TGTCGTTATAGCTGATAGTATTCCA	AGAATTACGATGTTTAGCCCTGA	36.0	39.1	57.2	57.6	2870-2871 bp	1.0	40.4
rpoC1_p4	GTCGTTATAGCTGATAGTATTCCA	ACGATGTTTAGCCCTGAATT	37.5	40.0	55.7	55.0	2863-2864 bp	1.0	40.4
rpoC1_p5	TGTCGTTATAGCTGATAGTATTCC	ACGATGTTTAGCCCTGAATT	37.5	40.0	55.7	55.0	2864-2865 bp	1.0	40.4