Hotspot

rbcL

Genome · 33883-35310 · 1428 bp

Back to Lycopodium

Pi 0.00354

MarkerSeek score 47.20

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: Genome
length_bp: 1428
variable_sites: 5
pi: 0.00354
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 47.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	GGGTCAGAAGGGATTCGAAC	TGGGCATCTTACCTGGAGAA	55.0	50.0	58.0	58.0	2425-2450 bp	1.0	80.9
rbcL_p2	GGGTCAGAAGGGATTCGAAC	TTGGGCATCTTACCTGGAGA	55.0	50.0	58.0	58.0	2426-2451 bp	1.0	80.9
rbcL_p3	GGTCAGAAGGGATTCGAACC	TGGGCATCTTACCTGGAGAA	55.0	50.0	58.0	58.0	2424-2449 bp	1.0	80.9
rbcL_p4	GGTCAGAAGGGATTCGAACC	TTGGGCATCTTACCTGGAGA	55.0	50.0	58.0	58.0	2425-2450 bp	1.0	80.9
rbcL_p5	GGTCAGAAGGGATTCGAACC	ACATCTAGGACTGGGCCAAT	55.0	50.0	58.0	58.1	2398-2423 bp	1.0	80.4