Hotspot

psbI-trnS-GCU

Genome · 77027-77102 · 76 bp

Back to Lycopodium

Pi 0.0263

MarkerSeek score 64.50

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: Genome
length_bp: 76
variable_sites: 2
pi: 0.0263
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	CAAACGATCCCGGACGTAAT	TAAAGCGGCGGATTGCTAAT	50.0	45.0	58.1	58.0	181 bp	1.0	80.0
psbI-trnS-GCU_p2	AAACGATCCCGGACGTAATC	TAAAGCGGCGGATTGCTAAT	50.0	45.0	57.8	58.0	180 bp	1.0	78.9
psbI-trnS-GCU_p3	CAAACGATCCCGGACGTAAT	GGGTTCGAATCCCTCTCTCT	50.0	55.0	58.1	58.2	137 bp	1.0	78.9
psbI-trnS-GCU_p4	CTATCAAACGATCCCGGACG	TAAAGCGGCGGATTGCTAAT	55.0	45.0	58.2	58.0	185 bp	1.0	78.7
psbI-trnS-GCU_p5	CAAACGATCCCGGACGTAAT	TTCGAATCCCTCTCTCTCCG	50.0	55.0	58.1	58.3	134 bp	1.0	78.1