Hotspot

trnF-GAA-trnL-UAA

Genome · 41227-42803 · 1577 bp

Back to Lycopodium

Pi 0.0108

MarkerSeek score 63.50

Primer available yes

Label trnF-GAA-trnL-UAA

Feature Metadata

label_name: trnF-GAA-trnL-UAA
feature_type: igs
region: Genome
length_bp: 1577
variable_sites: 17
pi: 0.0108
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-trnL-UAA_p1	TACCAACTGAGCTATCCCGG	GGTTTTCAGTGCTGATCCCA	55.0	50.0	58.6	58.1	1692-1694 bp	1.0	75.5
trnF-GAA-trnL-UAA_p2	TCCTCTGCTCTACCAACTGA	GGTTTTCAGTGCTGATCCCA	50.0	50.0	57.4	58.1	1702-1704 bp	1.0	75.4
trnF-GAA-trnL-UAA_p3	TACCAACTGAGCTATCCCGG	TCCGGTTTTCAGTGCTGATC	55.0	50.0	58.6	58.2	1695-1697 bp	1.0	74.7
trnF-GAA-trnL-UAA_p4	TCCTCTGCTCTACCAACTGA	TCCGGTTTTCAGTGCTGATC	50.0	50.0	57.4	58.2	1705-1707 bp	1.0	74.6
trnF-GAA-trnL-UAA_p5	TACCAACTGAGCTATCCCGG	CCCAGCAACAATGTGAATCG	55.0	50.0	58.6	57.7	1676-1678 bp	1.0	74.1