Hotspot

cemA-ycf4

Genome · 30367-30584 · 218 bp

Back to Lycopodium

Pi 0.0265

MarkerSeek score 64.40

Primer available yes

Label cemA-ycf4

Feature Metadata

label_name: cemA-ycf4
feature_type: igs
region: Genome
length_bp: 218
variable_sites: 5
pi: 0.0265
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA-ycf4_p1	TGGAGTGTCTGAAAACCACA	ATGGAAGAAAAAGCTGCCGA	45.0	45.0	56.9	58.1	303-332 bp	1.0	75.8
cemA-ycf4_p2	TGGAGTGTCTGAAAACCACA	TGGCTCGTTTCTTGCATGTA	45.0	45.0	56.9	57.8	281-310 bp	1.0	75.2
cemA-ycf4_p3	AGAACGAGATGGAGTGTCTGA	ATGGAAGAAAAAGCTGCCGA	47.6	45.0	58.2	58.1	312-341 bp	1.0	74.9
cemA-ycf4_p4	TGGAGTGTCTGAAAACCACA	CCGAATTGGCTCGTTTCTTG	45.0	50.0	56.9	57.8	287-316 bp	1.0	74.6
cemA-ycf4_p5	AGAACGAGATGGAGTGTCTGA	TGGCTCGTTTCTTGCATGTA	47.6	45.0	58.2	57.8	290-319 bp	1.0	74.3