Hotspot

rps12-rpl20

Genome · 17724-20886 · 3163 bp

Back to Lycopodium

Pi 0.0111

MarkerSeek score 54.20

Primer available yes

Label rps12-rpl20

Feature Metadata

label_name: rps12-rpl20
feature_type: igs
region: Genome
length_bp: 3163
variable_sites: 34
pi: 0.0111
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-rpl20_p1	CTCTGGAGGATCACACCCTT	CACAATTGCATCGGTTTTCA	55.0	40.0	58.4	55.5	200-211 bp	1.0	63.0
rps12-rpl20_p2	TGGAGGATCACACCCTTGTT	CACAATTGCATCGGTTTTCA	50.0	40.0	58.6	55.5	197-208 bp	1.0	62.0
rps12-rpl20_p3	TCTGGAGGATCACACCCTTG	CACAATTGCATCGGTTTTCA	55.0	40.0	58.7	55.5	199-210 bp	1.0	60.7
rps12-rpl20_p4	AGGATCACACCCTTGTTAGC	CACAATTGCATCGGTTTTCA	50.0	40.0	57.2	55.5	194-205 bp	1.0	60.2
rps12-rpl20_p5	GGATCACACCCTTGTTAGCT	CACAATTGCATCGGTTTTCA	50.0	40.0	57.2	55.5	193-204 bp	1.0	60.2