Hotspot

rpl16-rpl14

Genome · 5053-5185 · 133 bp

Back to Lycopodium

Pi 0.0376

MarkerSeek score 74.10

Primer available yes

Label rpl16-rpl14

Feature Metadata

label_name: rpl16-rpl14
feature_type: igs
region: Genome
length_bp: 133
variable_sites: 5
pi: 0.0376
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rpl14_p1	ATTGTGGCTGCACCTACAAA	TTAGCTCCACTGTTGTCTGC	45.0	50.0	58.0	57.8	231 bp	1.0	80.3
rpl16-rpl14_p2	AGCTGCGTACAAAATGCCTA	TTAGCTCCACTGTTGTCTGC	45.0	50.0	57.9	57.8	265 bp	1.0	79.3
rpl16-rpl14_p3	TAGCTGCGTACAAAATGCCT	TTAGCTCCACTGTTGTCTGC	45.0	50.0	57.9	57.8	266 bp	1.0	79.3
rpl16-rpl14_p4	GTGGCTGCACCTACAAAGAT	TTAGCTCCACTGTTGTCTGC	50.0	50.0	58.2	57.8	228 bp	1.0	79.0
rpl16-rpl14_p5	ATTGTGGCTGCACCTACAAA	GCTCCACTGTTGTCTGCTAC	45.0	55.0	58.0	58.6	228 bp	1.0	77.2