Hotspot

rbcL

LSC · 57206-58660 · 1455 bp

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Pi 0.00693
MarkerSeek score 35.40
Primer available yes
Label rbcL

Feature Metadata

label_name
rbcL
feature_type
gene
region
LSC
length_bp
1455
variable_sites
30
pi
0.00693
unique_haplotype_count
16
primer_available
yes
alignment_reliability
1.00
markerseek_score
35.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL_p1 GAGTTGTAGGGAGGGATTT TGGGCCGAGTTTAATTGCAA 47.4 45.0 53.4 58.4 1547-1574 bp 1.0 41.6
rbcL_p2 GAGTTGTAGGGAGGGATTT TTGGGCCGAGTTTAATTGCA 47.4 45.0 53.4 58.4 1548-1575 bp 1.0 41.6
rbcL_p3 GAGTTGTAGGGAGGGATTT ATTGGGCCGAGTTTAATTGC 47.4 45.0 53.4 57.0 1549-1576 bp 1.0 41.6
rbcL_p4 GAGTTGTAGGGAGGGATTT GGGCCGAGTTTAATTGCAAT 47.4 45.0 53.4 57.0 1546-1573 bp 1.0 41.6
rbcL_p5 GAGTTGTAGGGAGGGATTT AGTAAAAGATTGGGCCGAGT 47.4 45.0 53.4 56.8 1557-1584 bp 1.0 41.6