Hotspot

ndhG

SSC · 120046-120576 · 531 bp

Pi 0.00321

MarkerSeek score 30.70

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 8
pi: 0.00321
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 30.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	TTCGAATCAACCCCCTTCTG	CGAGGCCGAATACACTTTGA	50.0	50.0	57.5	58.0	1936-1975 bp	0.961538	77.6
ndhG_p2	TTCGAATCAACCCCCTTCTG	ATTGCGTTGAGTATTGCCCA	50.0	45.0	57.5	58.2	1770-1809 bp	0.961538	76.5
ndhG_p3	TTCGAATCAACCCCCTTCTG	AAATCGCTTTGGGTCGCTTA	50.0	45.0	57.5	58.2	1680-1719 bp	0.961538	76.5
ndhG_p4	TTCGAATCAACCCCCTTCTG	GCTTGTGAAGTATGTGTGCG	50.0	50.0	57.5	57.8	1900-1939 bp	0.961538	75.8
ndhG_p5	TTCGAATCAACCCCCTTCTG	TGTGAAGTATGTGTGCGTGT	50.0	45.0	57.5	57.8	1897-1936 bp	0.961538	75.8